無窮花의 成熟胚에서 體細胞胚 發生과 植物體 再分化

Somatic embryogenesis and plant regeneration from mature zygotic embryos of Hibiscus syriacus L.

Somatic embryogenesis and plant regeneration were obtained from mature embryos in Hibiscus syriacus L. ‘Bulsae’. Embryos were cultured on modified Murashige and Skoog(MS) or Woody Plant Medium(WPM) supplemented with 1,000mg/ℓ glutamine, 50mg/ℓ glycine and 50mg/ℓ serine, BAP(0 or 1.0mg/ℓ and 2,4-D(0.2~2.0mg/ℓ) in darkness. After 2 months, inoculated explants were transferred to MS medium without growth regulators and additives ; thereafter somatic embryos in various developmental stages were generated. When somatic embryos were transferred to half-strength MS medium containing 1.0mg/ℓ GA₃ and 1.0mg/ℓ ABA, they matured, germinated and produced plantlets with viable shoots and primary roots. Regenerated plantlets were hardened in a perlite:peatmoss(1:1, v/v) potting soil in culture room(25±1℃, 16/8 hour photoperiod) for 4 weeks, then transferred to greenhouse.