벼 葯培養에서 캘러스 形成과 植物體再分化能力에 대한 遺傳分析

Genetic analysis of callus formation and plant regeneration in anther culture of rice

Inheritance of callus formation and plant regeneration in rice anther culture was studied using the diallel technique. Anthers containing uninucleate microspores from four cultivars(japonica ; “Chuncheongbyeo” and “Palgongbyeo”, Tongil ; “Chilseongbyeo”, indica ; “IR50”) and their F₁’s were cultured on N₆-Y₁ medium supplemented with 1.0㎎/ℓ NAA and 2.0㎎/ℓ kinetin. The one-step culture method of anthers using the culture bottle was applied for standardization of anther culture process in this study. The parents showed significant differences in frequency of callus formation, green plant regeneration and albino occurrence. General and specfic combining abilities were estimated and found to be highly significant for the three traits. Reciprocal effect was also significant for callus formation and albino occurrence, but it was not significant for green plant regeneration. Further analysis for green plant regeneration showed gene action to be overdominant with high response being highly recessive and controlled by a few genes. The estimates of narrow sense heritability for albino occurrence(0.32) was lower than those of callus fromation(0.76) and green plant regeneration(0.55).