보리 葯培養에 前處理와 植物 生長調節劑 處理가 캘러스 形成 및 植物體 分化에 미치는 效果

Effects of pretreatment and plant growth regulator on induction of anther derived-callus and plant differentiation in barley

This study was carried out to find an efficient method of barley anther culture and to investigate the induction of anther derived-callus in accordance with durations of pretreatment at 5℃, stages of pollen development and the combined treatment of NAA and BAP. In the callus induction at 5℃ in the respective durations of pretreatment, it was shown that Deuce produced better calli at non-pretreatment while Duke and Dissa showed higher calli induction at pretreatment of 20 days and 10 days, respectively. The high callus responses in Deuce and Dissa were found at the early and mid uninucleated stage. In the callus reponses of the pollen treated with combinations of NAA and BAP, Deuce and Dissa responded well to 3.0㎎/ℓ NAA alone. The callus induced from anther culture largely produced albino plants and rarely produced normal plants. However, large callus was beneficial for inducing plants.