마 珠芽(零餘子) 器內培養에 의한 캘러스 誘起 및 植物體 再分化

Callus induction and plant regeneration from in vitro culture of dioscorea japonica tunberg bulbil

This studies was carried out to understand the effect of some kinds of media, concentration levels of plant growth regulators and activated characoal on callus induction plant regeneration and growth aspects of regenerated plant from bulbil in vitro culture. Percentage of callus induction was reduced at gradual increase levels of IAA concentration with activated charcoal MS compared with control MS. Regeneration frequency showed to increase at low level of IAA concentration with activated charcoal 1/8 MS in all culture varieties. In case of Jangma variety, callus induction was not showed at low level of IAA concentration with activated charcoal 1/8 MS. Regeneration frequency with the kinds of media were 86% at Heller, 85% at 1/8 MS and 80% at N6. In case of N6, number of roots and number of branch were remarkably increase at additive concentration level with IAA 0.5~1.0㎎/ℓ and kinetin 1㎎/ℓ, also in case of MS, percentage of callus induction was high, but regeneration frequency was very low.