벼 葯培養에서 低溫 前處理期間 및 培養條件에 따른 白色體發生

Effect of cold pretreatment and cultural conditions on albino frequency in rice anther culture

The frequency of albinos was significantly increased up to 72.2% to total pollen plants differentiated when panicles containing adequate pollen stage pretreated with 12℃ for 25 days. Genotypic difference in the frequency of albinos was not recognized, but the value of standard error in the frequency of albinos of japonica rice was lower than that of indica or Tongil-type rice. When cold pretreatment with 12℃ for 15 days applied, the frequency of albinos was low in 45 days old callus after anther inoculation compared to that of 55 days and 65 days. Combination of 2.0㎎/ℓ NAA, 1.0㎎/ℓ kinetin, and 5.0㎎/ℓ ABA in callus formation medium was effective to reduce up to 29% to total pollen plants differentiated even in 55 days old callus. There are no significant difference in the freuqency of albinos when cultural temperature range 24˚-28℃ through culture procedure was applied.