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학술저널

청피홍심무에서 캘러스 유도와 고 anthocyanin함유 세포주 선발

Callus induction and selection of high anthocyanin producing cell lines in Raphanus sativus L. cv. chungpihongsim

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Cotyledon was better than leaf, stem and root for callus induction and pigment synthesis in the callus cells. Anthocyanin biosynthesis was increased under dark condition when 2,4-D 0.5㎎/ℓ was added. Higher yielding callus cell line(ACL-1) was selected from the induced callus. A protoclone(APL-22) was selected, which grew fast and anthocyanin synthesis was enhanced by three times as compared with that of the ACL-1.

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