高磁氣場處理가 벼 幼苗生長과 培養細胞 再分化 및 蛋白質 二次元 電氣泳動패턴에 미치는 影響

Effects of high-magnetic fields on growth of rice seedlings and regeneration and protein pattern of cultured cells

Responses of rice seeds and cultured cells subjected to high magnetic field stress ranging from 0.5 kilogauss(k gauss) to 4.0 k gauss were determined. The growth of roots exposed to magnetic field at 4.0 k gauss was stimulated by 8.8 and 14% over that of the control, whereas shoots inhibited by 7.0 and 10% in dry and imbibe treatment, respectively. Treatments of magnetic fields at 1.0 and 4.0 k gauss significantly increased the regeneration ability of roots from the cultured cells up to 60.7 and 63.7%, respectively, as compared with untreated control showing 50.1% at 30 days after treatment. Some increased and newly synthesized polypeptides, particularly the size of 22.3 kilodalton and pI 7.0, in the cells exposed to magnetic field of 4.0 k gauss were identified using two dimentional polyacrylamide electrophoresis, demonstrating that cultured rice cells respond to high magnetic field stress by changing their protein patterns.