煙草의 葉肉原形質體로부터 再分化된 植物의 特性

Characterization of plants regenerated from mesophyll protoplast of Nicotiana tabacum L.

This experiment was conducted to investigate the variability of the plants(somaclones) regenerated from mesophyll protoplast of N. tabacum. Root tip chromosome numbers were determined in 137 unselected clones regenerated from mesophyll protoplasts of the flue-cured tobacco variety Bright Yellow 4. Seventy-seven percent of the plants retained the parental chromosome number of 2n=48 and the remaining plants were tetraploid. Randomly selected 28 clones among above diploids were analyzed for variability. Mean value of all investigated characters for 28 clones were not different significantly from parental variety. However, some clones differed significantly from parental variety in leaves per plant, days to flower, nicotine, total sugar, and nitrogen contents.