감자의 4x×2x 종간교잡 후대 식물체의 배수성 차이에 따른 氣孔細胞의 量的形質 變化와 반수체식물 선발효과

Stomatal cell characters as an indicator for haploid indentification from progeny plants derived by 4x×2x interspecific crosses in potatoes

To select haploids from the progeny plants obtained by interploidal and interspecific crosses of potatoes, stomatal cell characters were examined at three different growth stages as a potential indicator of ploidy level. The mean stomatal cell densities were 66.9, 45.0 and 38.5 per unit area(34 ㎛²) in haploid, triploid and tetraploid plants, respectively. The length and width of stomata were 27.5 : 18.8㎛, 34.3 : 21.0㎛ and 36.5 : 22.0㎛ in haploid, triploid and tetraploid plants, respectively. These characters had significant differences between ploidy levels with in the progeny plants but had relatively large quantitative variation within ploidy levels. On the other hand, the number of chloroplasts in a pair of guard cells, having the mean of 12.2, 18.4 and 20.2 in haploid, triploid and tetraploid plants, respectively, had large differences between ploidy levels with narrow variation within a ploidy level. These results indicated that counting chroloplasts in the guard cells among stomatal cell characters could be adopted as a rapid and accurate method for identifying haploids from large number of progeny plants of interploidal and interspecific crosses.