사시나무(Populus davidiana Dode)의 葉 切片 器內培養에 의한 植物體 再分化

Plant regeneration from leaf explants of Populus davidiana Dode

This study was performed to establish in vitro culture system of Korean aspen(Populus davidiana) using direct adventitous shoot regeneration. For the direct adventitous shoot regeneration from leaf explant, MS medium and the modified MS(m-MS) media composed of various combinations of BA and NAA were tested. For all three clones(Odae 13, Odae 15, Bonghyun 3) tested, m-MS medium containing 10mM ammonium(NH⁺₄), 20mM nitrate(NO⁻₃), 0.5㎎/ℓ BA and 0.1㎎/ℓ NAA was suitable for adventitious shoot regeneration form leaf explant of Korean aspen. Two forms of nitrogen sources, ammonium(NH⁺₄) and nitrate(NO⁻₃) were required for the direct adventitous shoot regeneration from excised leaf culture in this study. In addition, 30mM [10mM ammonium(NH⁺₄), 20mM nitrate (NO⁻₃)] nitrogen concentration among the four concentration tested was the best for direct adventitious shoot regeneration. When the NAA concentration in the m-MS medium was increased, polar regeneration was decreased. In the m-MS medium with 0.5㎎/ℓ and 0.2㎎/ℓ NAA, the percentage of shoot regeneration was 90% and the percentage of polar regeneration was 0%. As a result, this study showed that the form and concentration of nitrogen sources, and combination of BA and NAA affect greatly to the adventitious bud regeneration of leaf explant culture. Besides, the tissue culture technique developed in this study will be useful tool for the application of foreign gene transformation using Agrobacterium binary vector system.