보리再培種과 野生種(hordeum bulbosum) 間 雜種 幼胚의 發育增進

Improvement of embryo development in hybridization between hordeum vulgare and hordeum bulbosum

The purpose of this study is to investigate effects of materials and colors of crossing bags, removal of florets in a spike on formation and development of seed set and embryos, and to observe development of haploidy and diploidy embryos at different days after pollination for bulbosum method. Cellophane, polyethylene or paper was not suitable for crossing material, because the florets bagged with those materials turned to yellow and died not long after pollination. The humidity within the bags dropped too low when cellophane or paper was used, due to their porosity. While it rised too high upto dew point when polyethylene was used for its air-tightness. Double bags of parchment(16㎝ long)+ polyethylene (10㎝ long) which kept the temperature inside bags around 25℃, gave the best rates of formation and development of the embryos. The parchment inside functioned as a buffer against moisture by absorbing and releasing water properly. Path analysis with temperature, moistutre and light intensity inside the bags indicated moisture was the primary factor mostly influencing the embryo development. Removal of rows in a spike as a means of thinning out florets was not effective to improve development of embryo substantially. From the comparative observation on the haploid development of the seed set by H. bulbosum pollinator with diploid those set by selfing, it was noticed that the former had much smaller embryos, lacking morphologically differentiated organs in 14 days after pollination. The endosperm which had few starch particles in 11 days, was completely liquidified in 14 days after pollination.