Acifluorfen 抵抗性 토마토(lycopersicon peruvianum mill) 細胞株 選拔 및 植物體 分化

In vitro selection and regeneration of acifluorfen-tolerant cell lines of lycopersicon peruvianum mill

Fifteen acifluorfen-tolerant cell lines of Lycopersicon peruvianum were isolated by stepwise selection in suspension cultured cells. Growth of unselected line was completely inhibited at 0.5μM, while selectd callus lines grew at 8μM acifluorfen. After subculturing on acifluorfen-free medium for 5 passage(over 4 months), eleven cell lines tested maintained their tolerance to 0.5μM acifluorfen. The regeneration capacity of acifluorfen-tolerant cell lines differed depending on the cell line. Seven of 14 cell lines regenerated the shoot and six cell lines did not. Selected cell lines were more effective than the unselected control callus in regenerating on medium containing acifluorfen. The unselected callus of L. peruvianum did not regenerate shoots on medium containing over 1μM acifluorfen, but selected lines regenerated shoots on medium with 5μM acifluorfen. The addition of 10㎎/L spermidine to the medium improved the regeneration rate and the growth of acifluorfen-tolerant lines.