柴胡(bupleurum falcatum L.) 花粉粒의 形態的 特性

Morchological characteristics of pollen grain in bupleurum falcatum

Morphological variation was investigated in pollen grains of Bupleurum falactum collected from Korea and Japan through optical and scanning electron microscope(SEM) to get basic informations for varietal classification and ecological evolution. Pollen-dimorphism of Bupleurum falcatum was different according to the collected region. The ratio of S(small) pollen was highest in Akiyoshidai(23.4%) and E(empty) pollen ratio was highest in Ebino line(24.8%). Pollen grain shape of Buplerum falcatum was distinguished as three types ; that is, Korean collection was prolate, while that of Tsukuba-cultivated one was sub-prolate and that of Nara collection was prolate-spheroidal shape. When we observed the Bupleurum falcatum pollen grain through the SEM, critical point drying method was better for intact maintenance of original pollen shape compared with air drying treatment.