고추 배양조직에 있어서 NaCl이 묘생장과 분화에 미치는 영향

Effects of NaCl on seedling growth and induction of shoot in cultured tissue of Capsicum annuum L.

The induction of adventitious shoots from scarlped cotyledon of Capsicum annuun L. was investigated with the various combinations of BA and IAA levels, Up to 35 adventitious shoots were obtained per cotyledon on MS medium with 10 μM IAA and 12 μM BA, and 64 shoots per cotyledon were induced when 0.15% of peptone was added to this medium. The induced shoots rooted on 2/3 MS medium and were regenerated into whole plants. In order to examine the effects of NaCl on the stem elongation, and leaf and root expansion of seedlings and shoot induction from cotyledon, the seedlings were transferred to MS medium with 0~150 mM NaCl. After 3 and 6 weeks of culture, the growth of leaf, stem and root was completely suppressed on MS medium with 130 mM NaCl. Thus, around 130 mM NaCl seems to be limiting concentration for seedling growth and induction of shoot. To investigate the changes in soluble proteins caused by salt stress during shoot induction, soluble protein extracted from adventitious shoots developed from cotyledons by culturing on media with 0, 40, 80, or 120 mM NaCl was subjected to SDS-PAGE. As concentration of NaCl increases, 23, 22.6 and 21 KD that had low activity in the control without NaCl became more active. The proteins of 21.7KD and 20.6KD that had high activity in the control without NaCl showed a decreasing trend as the concentration of NaCl increases, but 26.4 KD remained at the similar level regardless of change in NaCl level.