Hordeum bulbosum 을 利用한 半數性 보리의 同定

Authentication of barley haploids in H. vulgare through pollination with H. bulbosum

This study was conducted to authenticate barley haploids by cytological, electrophoretical and morphological observations in order to establish an effective bulbosum method. Somatic chromosome numbers were 7 in the suspected barley hybrids compared to 14 in both H. bulbosum and H. vulgare, even though the chromosomes of the two species were not exactly distinguished. Barley haploid was clearly shown because of the different isozyme patterns of the two species, and suspected barley haploids. The colchicine-induced diploids with H. bulbosum chromosomes always displayed several characteristics of H. bulbosum, especially prostrate habit of growth. Progeny of the diploids was morphologically identical to their seed parents, which was the additional proof that they were diploids of genetically complete barley haploids. Genotypic variation for haploid embryo formation in eleven selected Korean barley cultivars ranged from 15 to 52%, and the variation for plant development from embryo culture ranged from 18 to 55%. H. bulbosum pollinator, GBC gave a little better results for embryo formation and plant development than Sp. D. did.