알로에(aloe vera L.) 生長點培養에 있어 生長調節物質 및 培地가 植物體分化와 生長에 미치는 影響

Effect of growth regulators and media on regeneration and plant growth in meristem culture of aloe vera L.

This study was conducted to investigate the optimum medium and levels of growth regulators for multiple shoot induction as a rapid micropropagation system from meristem culture of Aloe vera L. in vitro. MS medium supplemented with 3㎎/ℓ BA was effective for shoot regeneration and addition of 0.1μM TDZ increased the number of shoots but retarded the growth of shoot. More shoots were regenerated when 2㎎/ℓ BA combined with 0.1㎎/ℓ IAA than when only cytokinin were added onto the medium. MS medium containing IAA and NAA was effective for rooting compared with medium containing no growth regulators. When regenerated plantlets were transplanted on pot, the survival rate of plants was 75% on perlite and 95% on vermiculite, respectively.