라이밀의 葯培養 適期 判定을 위한 指標特性

Morphological indicator for optimal stage of microspore in triticale anther culture

Optimum stage of microspore development and its best indicator for triticale anther culture were investigated by using triticale cv. “Sinkihomil”. The length from the auricle of flag leaf to the top of spike was the best indicator for determining optimum stage of microspore development. During the pretreatment at 5℃, the position of the top of spike was increased by 3~4㎜ a day and the microspore was slightly developed. It was observed that the early uninucleate stage of microspore was best for callus formation. Calli induced from microspores at early and early-mid-uninucleate stages were regenerated a lot of green plants, while albino plants were increased on calli obtained from late-uninucleate microspores.