薄荷 懸濁培養에서 培地 濃度와 spermidine의 細胞生長, 精油含量 및 精油成分에 미치는 影響

Effect of medium strength and spermidine on cell growth, essential oil production and its composition in suspension culture of Mentha piperita L.

This experiment was carried out to know the effects of medium strength and spermidine on cell growth, essential oil production and its composition in Mentha piperita cell suspension cultures. The results indicated that half strength of modified Lin-Staba medium(potassium phosphate 170㎎/ℓ, sucrose 20g/ℓ, ratio of ammonium nitrogen to nitrate=1 : 3) was the most effective on cell growth and oil production. But menthol content was higher in full strength of modified Lin-Staba medium. Concentration of 10㎎/ℓ spermidine showed positive effect on both cell growth and essential oil production. Menthol and menthone content were increased more than two times compared with the control when 10㎎/ℓ spermidine was added.