들깨 葯培養에서 低溫 및 生長調整劑 處理가 캘러스誘導와 植物體 再分化에 미치는 影響

Effect of low temperature and plant growth regulators on callus induction and plant regeneration in anther culture of perilla

This experiment was carried out to determine the effect of low temperature pre treatment and plant growth regulators on callus induction and plant regeneration in perilla anther culture. Perilla panicle with floral envelope was pretreated in low temperature at 5℃ and l0℃ for three days and compared with contral. MS basic medium supplemented with 2, 4-D 1~5㎎/ℓ, NAA 1~3㎎/ℓ and kinetin 2~5㎎/ℓ was used for callus induction and MS medium supplemented with NAA 0.1㎎/ℓ and BA 0.5㎎/ℓ was used for plant regeneration. Cold treated anthers at 5℃ for three days induced more callus and plant regeneration than others. In percentage of callus induction, MS medium supplemented with 2, 4-D ㎎/ℓ, NAA 1㎎/ℓ and kinetin 2㎎/ℓ induced more callus than N6 and B5 medium supplemented with the same concentration of plant growth regulators. The percentage of plant regeneration of Yeopsil×Jukjaso (F₁) in MS medium supplemented with 2, 4-D 5㎎/ℓ, NAA 3㎎/ℓ and kinetin 5㎎/ℓ was about 63%, while that in MS medium supplemented with 2, 4-D 2㎎/ℓ, NAA 1㎎/ℓ and kinetin 2㎎/ℓ was about 32%. The percentage of plant regeneration of suwon 32 showed the same tendency at the same condition in the case of 10℃ pretreatment for three days on Yeopsil×Jukjaso(F₁).