Uniconazol처리로 誘起된 옥수수 遺傳子 ZmKT4과 ZmKT28의 cloning 및 sequencing

Cloning and sequencing of genes(ZmKT4 and ZmKT28) induced by uniconazol treatment in maize

The seedling obtained from uniconazol treatment showed dwarfism and tillering. In order to identify uniconazol-induced genes, cDNA hybridization probes were made from poly(A⁺) RNA isolated from uniconazol-treated seedlings and untreated inbred line seedling(IK), and the putatively regulated clones was detected by the differential screening of the cDNA libray. RNA hybridization experiment revealed that two of the clones were in fact induced by application of uniconazol. mRNAs of ca. 800 and 1,500 baseds was identified by isolated two clones (ZmKT4 and ZmKT28) and markedly increased from 48hr after uniconazol application. ZmKT4 clones showed the highest level of mRNA accumulation in tassels, ears and leaves, and the lowest level in roots. ZmKT28 clone is expressed in the stems, tassels, silks, ears and leaves but no transcripts was detected in roots. Two clone may be associated wit the cell elongation and phase of vegetative growth.