RAPD法을 利用한 수박의 遺傳變異 探索

Genetic diversity in watermelon(Citrullus vulgaris L.) germplasm through RAPD analysis

A total of 39 genotypes of watermelon were chosen to identify the frequency of RAPD polymorphisms and to group into sub-populations. Of the 15 primers tested, 14 primers except primer 275 showed the polymorphic pattern. A total of 162 bands were generated across all 39 genotypes. Among them, 100(62%) were polymorphic and the remaining 62(38%) were monomorphic. The mean 1-F(genetic distance) was 0.114, and this value corresponded to the mean value of 0.117 detected in 36 genotypes of sorghum. The hightest value was 0.309 and the smallest one was 0.012. The 100 polymorphic fragments of total 162 bands were proved to be very reliable and confident, and selected as useful polymorphic markers. The mean value in this comparison was 0.24 and the highest onewas 0.69. Eight RAPD markers were expected to be utilized in the unique variety discrimination of eight watermelon genotypes. Using DNA polymorphisms, the two major clusters were resolved and one of these was subdivided into three cluster. Each sub-group were characterized and identified again with morphological and genetic characteristics of respective genotype.