韓國 自生 茶나무의 RAPD-marker에 의한 類緣關係

Genetic relationship among Korean native tea trees(Camellia sinensis L.) using RAPD markers

Genetic relationship and variation in Korean native tea(Camellia sinensis L.) were analysed using RAPD-PCR markers. Total 19 primers were used and 116 bands of the amplified DNA bands were generated across all 35 collections. Among the obtained bands, 18 of them were monomorphic(15.5%) and the others appeared to be reliable polymorphic(84.5%) marker. Genetic distances(GD) based on the 116 RAPD bands were used to construct phylogenetic dendrogram based on analysis of band sharing data of amplified markers. GDs of them were ranged from 0.070 to 0.367, and the mean value was 0.155. Local collections from same areas of Beknyon-am and Pyagol showed relatively lower GDs(0.122, 0.102, respectively), which infer genetically less divergent within those areas. Local collections from Sunun-sa area showed higher genetic distance(0.222). In genetic relationship based on complete-linkage clustering, the tea collections were classified into three group, A group(A-1, A-2, A-3), B group(B-1, B-2) and C group. It assumes there is extremely lower genetic difference within group. In genetic evolutionary pattern based on Neighbor-joining clustering, the tea collections with similar variation were divided into A group(A-1, A-2), B group(B-1, B-2) and C group(C-1, C-2).