벼 雄性不稔系統의 葯 採取時期 및 不稔細胞質이 葯培養에 미치는 影響

Effect of pollen-development stage and sterile cytoplasm on anther culture of male-sterile rice plants

Anther culturability of CGMS(Cytoplasmic and genetic male-sterile) lines was studied in relation to the pollen-development stage and the effect of sterile cytoplasms in Japonica rice. Callus induction rates of CGMS lines were 45.2%~61.8% in COA-ms and 4.1%~8.1% in WA-ms. Regeneration frequencies ranged from 20.2% to 27.7% without any particula difference between two different cytoplasmic male-sterile lines. Gwanakbyeo and Gwanak COA-ms showed the same callus induction ability along evry pollen-development stages. Callus induction rate of Gwanak WA-ms anther in early uninucleate stage was similiar to that of Gwanakbyeo, however, that was drastically decreased after the stage. It was discussed that sterile pollens caused by sterile cytoplasms were different from S- or E-pollens occuring in normal plants in response to anther culture. Sterile cytoplasms of COA-ms and WA-ms did not affect directly the callus induction and plant regeneration. The regenerated plants derived from anther culture of MS lines were all male-sterile.