마카로니(durum)밀의 三染色體植物을 이용한 esterase isozyme 遺傳子의 染色體位置 同定

Chromosomal location of esterase isozyme gene in durum wheat using trisomics

This study was carried out for identification of esterase isozyme polymorphism which may be an useful way to understand the physiological and biochemical characteristics in durum wheat, Triticum durm var. hordeiforme(2n=4x=28 AABB). The esterase isozyme was detected by electrophoretic separation and activity staining techniques with the endosperm sample from germinated durum wheat grain. Artifical octoploid showed nine isozyme bands which were designated as Ei-1, Ei-2, Ei-3, Ei-4, Ei-5, Ei6, Ei-7, Ei-8 and Ei-9 from cathode side. Normal tetraploid plant(2n=4x=28) showed six bands, without bands of Ei-6, Ei-8 and Ei-9 in durum wheat. Only six bands such as Ei-1, Ei-2, Ei-3, Ei-4, Ei-5 and Ei-7 was detected in Tri-1A(2n=4x=29 AABB+11A) line, but the activity of isozyme in Tri-1A line was above double one of normal plants. Therefore, the gene(s) controlling six bands of esterase isozyme was considered to be located on the chromosome 1A. Moreover, the activity of six esterase isozyme bands in Tri-1B(2n=4x=29 AABB+11B) line was same as normal plants, but nine bands including Ei-6, Ei-8 and Ei-9 were detected in esterase isozyme of Tri-1B line. These three bands appeared in artifical octoploid(2n=8x=56 AAAABBBB) and did not appeared in normal plants or other trisomic lines, except Tri-1B line. Consequently, the gene controlling these three bands was considered to be on the chromosome 1B.