콩 未熟子葉으로부터 體細胞胚의 發生과 植物體 分化

Somatic embryogenesis and plant regeneration from in vitro cultured immature cotyledons of soybean

Immature cotyledons of soybean, Glycine max (L.), were cultured to improve the efficiency of somatic embryogenesis. Varietal differences for the capacity of embryo formation were evaluated to select a promising cultivar with high efficiency of somatic embryogenesis. Embryogenic capacity was strongly influenced by genotypes of donor plants. A Korean soybean leading cultivar, Danyeobkong, was identified to have the highest frequency(28.7%) of somatic embryogenesis among nine soybean accessions. Efficiency of embryogenesis was also varied with the developmental stages of soybean seed serving as explant sources. The maximum frequency(16%) of embryogenesis was obtained from the immature seeds with size of 4~6㎜. Addition of 2, 4-D in the medium enhanced somatic embryogenesis from immature cotyledons, but it inhibited the formation of normal somatic embryos. The induction frequency of somatic embryo with normal morphology was higher in the presence of NAA(10㎎/L) than that of 2, 4-D. Most of somatic embryos formed on medium with 2, 4-D were developed to abnormal types. Somatic embryogenesis from immature cotyledons was promoted in the presence of 3% sucrose.