TLC 方法에 의한 우리나라 在來鍾 香草의 cis-abienol 遺傳分析

Genetic analysis of cis-abienol in Korean local variety “Hyangcho”(N. tabacum L.) using TLC method

This study was conducted to obtain the information for breeding of aromatic flue-cured tobacco, and to select cis-abienol producing lines from the cross of NC82×Hyangcho. Parental genotypes were Hyangcho which produces cis-abienol and NC82 which doesn’t produce cis-abienol on the leaf surface. The parents, F₁, F₂, BC₁, and BC₂ generation were evaluated for cis-abienol production to determine the inheritance of cis-abienol on the leaf surface. The presence of cis-abienol on the leaf surface was determined using thin layer chromatography(TLC) method. Segregation patherns from F₂ and BC₂ generation revealed that presence or absence of cis-abienol on the leaf surface was controlled by alleles at a single locus. Cis-abienol was produced on leaves of the homozygous dominant and heterozygous genotype plant, while the homozygous recessive genotype plants did not produce cis-abienol.