人蔘 胚培養에 있어서 花器出現 特性

Characteristics of in vitro flower emergence in embryo culture of Panax ginseng C. A. meyer

This study was carried out to examine the path way of in vitro flower through embryo culture of Korean ginseng(Panax ginseng C. A. Meyer). Zygotic embryos were cultured on Murashige and Skoog(MS) medium with various concentrations of indolebutyric acid(IBA), benzyladenine(BA) and gibberellic acid(GA). There were two pathway of differentiations from the adventitious buds and shoot primordium in relation to the in vitro flower development. In this case, there are three types of flower emergence : shoot only, flowers only and shoot with flowers. When the amount of in vitro flower differentiation increased, the anther development was poor. The primary culture at 18℃ and the secondary culture at 25℃ under light condition was optimum for the in vitro flower induction.