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학술저널

Glycine屬 種間交雜 親和性과 未熟胚珠培養

Cross compatibility and immature ovule culture of interspecific hybrids in genus Glycine

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This study was carried out to investigate the cross compatibility, fertilization and immature ovule culture to gain interspecific hybrid plants between perennial and cultivar in Glycine. 1. Pod survival rate determined perennial Glycine tomentella hihger than Glycine max(L.) as a maternal parent and cross compatibility with cultivar investigated G. tabacina was lower than G. tomentella. 2. Immature ovule culture showed 22% of in virto germination, 4% of callus formation, 71% of no response, and succeeded two normal hybrid plantlets cultured on B5 basal medium supplemented 0.1㎎/ℓ BA and 0.2㎎/ℓ BAP. 3. In observation of initial fertilization after interspecific cross of Glycine, pollen tube germination on stigma and double fertilization were progressed more faster in G. tomentella/G. max than in G. max/G. tometella.

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