RAPD를 이용한 보리×밀 속간교잡종의 보리유전자 도입 확인

Detection of barley genes in barley×wheat intergeneric hybrids using random amplified polymorphic DNA(RAPD)

Random amplified polymorphic DNA(RAPD) was employed as a genetic marker in order to verify the genetic introgression of barley(cv. Manker 16) to barley × wheat backcross(BC₂F₁) lines. In RAPD analysis of 12 random primers tested, four of the 14 progenies obtained from barley × wheat backcrosses expressed band unique only to barley using primer number 225 (5’-CGA CTC ACA G-3’), and all progenies except H6-2 and H6-3 expressed bands unique only to barley using primer number 274(5’-GTT CCC GAG T-3’). All progenies of the BC₂F₁ lines appeared to have at least one of barley polymorphic patterns through RAPD analysis. Somatic chromosome numbers of BC₂F₁ lines ranged between 46 to 49 and appeared to have 4 to 7 chromosomes of barley. Use of RAPD as a genetic marker for the detection of genetic introgression of barley in barley × wheat intergeneric hybrid derivatives was effective and useful.