라이밀 葯培養에서 煎處理 條件이 캘러스 誘起에 미치는 影響

Effect of pretreatment condition on callus formation in triticale anther culture

Improvement of triticale anther culture techniques is essential to develope new varieties in limited time. This study was carried out to increase the efficiency of anther culture for haploid breeding in triticale. Before explanting, each solution of 2, 4-D, GA₃ and EACA(E-Aminocaproic Acid) was injected into the sheath with spike. GA₃ was more effective for callus induction than others. Also, pretreatment of explants for 3 to 7 days at 5℃ in C17, B5 and MS liquid media were better for callus induction. The spikes were treated with mutagens, X-ray and EMS(Ethylmethanesulphonate), just before inoculating anthers on C17 medium. The rate of callus formation increased in the anthers treated with 50R and 100R of X-ray, and 0.05, 0.1 and 0.2M of EMS.