In vitro culture condition for proliferation of rhizomes derived from cymbidium crosses

Rhizomes derived from seeds obtained from the crosses of C. kanran ‘Jeju’×C. goeringii and C. kanran ‘Jeju’×C. C. kanran ‘Namkuk’ were cultured in different media under different conditions in order to overcome the problems associated with shoot and root proliferation. The culture medium most suitable to induce organogenesis from rhizome culture of Cymbidium must be supplemented with 1.0㎎/ℓ BAP+0.3㎎/ℓ IAA. Furthermore, a supplement of 5% sucrose for two weeks followed by reduction to 2 to 4% concentration showed enhanced organogenesis. A light intensity of 3000lux and a type of closure of culture vessels which consited of curved glass tube plugged with cotton as well as 50㎛ nylon sieve also induced better growth. However, adding activated charcoal to the culture medium did not seem to stimulate rhizome growth and subsequent organogenesis. Five percent sucrose treatment for 2 weeks followed by reduction to 2 to 4% sucrose. However, adding activated charcoal into the culture medium did not seem to stimulated rhizome growth and subsequent organogenesis. These results provide basic information necessary for production of quality plants in large quantities by clonal multiplication and establishment of hybrid plants in Cymbidium.