Detecting DNA markers by RFLP analysis and DNA-fingerprinting in capsicum

This experiment was carried out to detect of cultivar specific markers using RFLP analysis and DNA-fingerprinting in Capsicum genus. The result of SDS-PAGE analysis could not detect genotype specific protein markers workable within each species of Capsicum, it however was possible to detect some interspecies markers. For RFLP analysis and DNA-fingerprinting, DIG labelled (GATA)₄, 26S rRNA, rice chloroplast DNA clones (P1, P2, P10-1, P45, P50, B3), and maize mitochondrial DNA clone (ATP A) were used as a probe. With a (GATA)₄ probe the level of polymorphism detected was high enough to differentiate all 29 genotypes used in this study. The results with the most informative banding patterns were obtained with the (GATA)₄ probe hybridized to Hae Ⅲ, Rsa Ⅰ, and 26S rRNA probe hybridized to BamH Ⅰ, Hinf Ⅰ, Hae Ⅲ, Hind Ⅲ resticted genomic DNA, respectively. Hybridization with the P2 probe which produced polymorphic bands (11kb) in only Masanjere by HindⅢ digest. Mitochondrial ATP A probes revealed three variable band patterns among the 29 red pepper genotypes digested by Hind Ⅲ and HaeⅢ. A same band patterns was obtained between X-mas bell Ⅰ and X-mas bell Ⅱ on a HindⅢ digest. In case of Green 100 genotype specific band detcted by HindⅢ, HaeⅢ digested. Subsequently, the multilocus probe, (GATA)₄, 26S rRNA and ATP A probes has proven successful in distingulishing between different red pepper genotypes.