RAPD를 이용한 마늘의 유연관계분석

Analysis of genetic relationship by RAPD technique in Allium sativum L.

To analyse the genetic relationship among garlic accessions(Allium sativum L.) the polymerise chain reaction(PCR) was performed with total genomic DNAs of 13 garlic accessions by random 14 primers. The genetic diversity and genetic distance among 13 garlic accessions were used to generate a dendrogram showing phylogenic relationship. Thirteen garlic accessions were classified into two groups(group Ⅰ, Ⅱ). In additon, one of the two groups Group Ⅰ was divided into three sub-groups(group Ⅰ-a, Ⅰ-b, Ⅰ-c). Group Ⅰ included garlic accessions of Hongcheon, Gangwha, Yeongdong, Cheongju, Danyang, Gochang, Euesung, Yaechun, Seosan, Samcheog and China, while Group Ⅱ included garlic accessions of California and Austria. Sub-group Ⅰ-a included garlic accessions of Cheongju, Euesung, Yaechun, Seosan, sub-group Ⅰ-b included garlic accessions of Gangwha, Yeongdong, Samcheog and sub-group Ⅰ-c included garlic accessions of Hongcheon, Danyang, Gochang, China, respectively.