陸地棉, 亞細亞棉 및 種間雜種의 胚珠培養

Immature ovule culture in hybrid of Gossypium hirsutum and G. arboreum

This experiments were conducted to establish ovule culture method for Gossypium hirsutum, G. arboreum and their hybrids to overcome difficulty to interspecific hybridization due to the ovule abortion in artificial crosses. In the ovule culture of Gossypium hirsutum and G. arboreum the percentage of survival ovule and callus formation in four weeks after culture was 45.6% in G. hirsutum and 37.3% in G. arboreum using MS medium including casein hydrolysate 250㎎/ℓ, IAA 5.0μM and kinetin 0.2μM concentration. The Schenk-Hildebrandt medium was superior to Beasley-Ting or Murashige-Skook ones for ovule culture of interspecific hybrid. Schenck-Hildebrandt medium added by LAA 5μM, Kinetin 0.2μM concentration showed the highest in the rate of survival embryo and plant formation with 44.4% and 2.9% in G. hirsutum×G. arboreum hybrids and 20.1%, 1.5% in the reciprocal hybrids, respectively. Both cotton species showed higher embryo survival and shoot formation in ovule culture (SH medium, 28℃) under the first fifteen days dark and then 16 hours condition light/8 hours dark conditions compared with continuous dark or light treatments. The hybrid ovules dissected out at four days after interspecific pollinations revealed high embryo survival and shoot formation.