RAPD 標識因子를 이용한 밀 稈長 近同質 系統의 遺傳變異 分析

Phylogenetic analysis of wheat near-isogenic lines for culm length with RAPD marker

RAPD markers were selected and used for grouping of 32 culm length near-isogenic lines of wheat(Triticum aestivum L.). Among a total of 147 bands observed by using 12 random primers, 113(77%) bands were polymorphic. Thirty-four bands were selected as reliable markers. The average marker difference was 8.6 among the 32 lines with the largest of 19 and smallest of 1. Genetic relationship based on the marker difference was identified using UPGMA computer program. The 32 lines could be grouped into two major clusters and then each of two groups subdivided into two sub-groups. This result corresponded to morphological and genetic characteristics of culm length near-isogenic lines of wheat. Seven specific DNA markers and five specific DNA markers were selected for discrimating the genetic background of lines between groups and within a group, respectively.