동위효소 및 RAPD 분석에 의한 소나무 천연집단의 유전변이 비교

Comparison of genetic variation in pinus densiflora natural populations by allozyme and RAPD analysis

Pinus densiflora, a diploxylon pine, is widely distributed in Korea, Japan, southern Manchuria and Santhung peninsula of China and plays very important roles in forestry and ecosystems. In this study, we have examined the genetic diversity and structure of P. densiflora, by comparing the patterns of variation at 23 allozyme loci with those of 31 RAPD loci investigated among 6 natural populations occupying similar geographic regions. On average, the level of genetic diversity at RAPD loci was greater than that of allozyme loci. The number of alleles per locus (A/L), the proportion of polymorphic loci (P) at 95% level, the observed (Ho) and expected (He) heterozygosities at allozyme loci were 2.3, 66.2%, 0.264 and 0.261, respectively while the values for A/L, P, Ho and He at RAPD loci were 2.0, 94.6%, 0.514, and 0.392, respectively. The degree of genetic differentiation was low (FST=0.032 and GST=0.031) at allozyme loci; however, the degree of differentiation at RAPD loci was much higher (FST=0.109 and GST=0.105). The populations close geographically did not show the tendency of clustering into the same group both at allozyme and RAPD loci. Finally, the genetic and statistical differences of both genetic markers (allozyme and RAPD) were discussed.