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학술저널

땅콩 水分含量 및 감마線量에 따른 突然變異 反應 差異

Mutagenesis of peanut(Arachis hypogaea L.) as affected by moisture content of seed and gamma-ray dosage

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In order to obtain the basic information on the mutation breeding in peanut and to find out the efficient methods for the induction of mutation and selection criteria, the seeds of peanuts were subjected to mutagen treatment with the various levels of the γ-ray dosages (10, 20, 30 and 40kR) in the seeds contained different moisture. Emergence and survival rate of M₁ plants were decreased by the increase of dosage of gamma rays and the lethal dose (LD₅₀) was 30-40kR in the dried peanut seeds. The higher radiation dose level and moisture content in seeds lead to the more radiosensitivity in M₁ generation. The lethal dose (LD₅₀) of peanut seed with 10-25% moisture content was around 20kR. The order and spectra of chlorophyll deficient mutations were maculata, chlorotica, chlorina, albina and the total chlorophyll-deficiency rate was 14.8% at 30kR dose in the cotyledonary branch. The chlorophyll deficieny rate was negatively correlated with the main stem length and number of pods per plant in M₁ generation. The highly positive correlation coefficients between chlorophyll deficieny rate and morphological variant rate was observed in M₂ population

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