生長調節物質이 벼 葯培養에 미치는 影響

Effects of growth regulators on rice anther culture

This study was carried out to investigate the effects of growth regulators on the microspore surival rate, pollen dimorphism, microspore-originated calli formation and plant regeneration. The anthers of rice were cultured on the N₆ basal medium supplemented with the single or the combination of α-naphthalen acetic acid (NAA), 2, 4-dichlorophenoxy acetic acid(2, 4-D) and Kinetin. Microspore surival rate was not greatly different among growth regulators in 5 days of culture, but it was the highest on the medium with 1 mg/L NAA(81.9%), and the lowest on the medium with 4 mg/L Kinetin (40.3%) in 20 days of culture. Appearance rate of S pollen was low on the medium with 4 mg/L, Kinetin (24.2%), and high on the medium with 1 mg/L NAA (75.6%) Both of callus induction and plant regeneration were good on the medium supplement with NAA or 2, 4-D each, the combination of NAA with Kinetin, and 2, 4-D with Kinetin. The rate of induction was best on the medium with 1 mg/L 2, 4-D (55.1%). But the plant was not differentiated in calli induced from the medium with only Kinetin supplemented. The frequency of plant regeneration was excellent in calli induced from the medium with 2 mg/L NAA (13.5%)