Diversity of restriction fragment length polymorphism in soybean

The assessment of the degree of restriction fragment length polymorphism (RFLP) may facilitate construction of RFLP linkage maps. For the four parents derived from two soybean populations, ‘Suwon 157’x‘Danbaekkong’ and ‘Pureunkong’x‘Jinpumkong 2’, the degree of RFLP was assessed by screening parental genomic DNA digested with each one of five restriction enzymes (DraⅠ, EcoRⅠ, EcoRⅤ, HindⅢ, or TaqⅠ) using cDNA and/or genomic clones from five legume species (Glycine max, Vigna radiata, Phaseolus vulgaris, Arachis hypogaea, and Medicago sativa). The restriction enzymes, EcoRⅠ and HindⅢ, detected higher polymorphisms between parents in both populations. Comparatively higher polymorphisms were found using DNA probes from G. max, A. Hypogaea, and P. vulgaris. Of 246 DNA probes, 58% detected polymorphisms between ‘Suwon 157’ and ‘Danbaekkong’, and 51% between ‘Pureunkong’ and ‘Jinpumkong 2’, indicating the higher level of polymorphisms. This higher polymorphism in both populations is probably due to the larger amount of genetic diversity between soybean genotypes evaluated in this study.