목화 陸地棉과 亞細亞棉 種間雜種의 胚珠培養時 生長調節劑와 炭素源의 影響

Effects of growth regulators and carbon sources on ovule culture of interspecific hybrid between Gossypium hirsutum and Gossypium arboreum

This study was performed to increase the efficiency of interspecific hybrid production between Gossypium hirsutum and Gossypium arboreum via in vitro ovule culture. Immature ovules dissected from the interspecific crossed cotton fruit at 4 days after pollination were cultured in Shenck-Hilderbrandt(SH) liquid medium supplemented with various growth regulators and carbon sources. The best results were observed from the combination of 5mM IAA, 0.005mM GA, 0.05mM Kinetin with 18.5% of embryo survival and 10.6% plant regeneration. Glucose and sucrose were considered as reasonable carbon sources for the ovule culture of cotton achieving the highest frequency of plant regeneration at 150mM and/or 200mM, respectively.