RAPD 방법을 이용한 벼의 유연관계 분석

Analysis of genetic distance using RAPDs in rice

Random amplified polymorphic DNAs (RAPDs) were identified in rice by amplification using 10-mer primers of arbitrary sequence. The number of amplification products increased with increasing GC content of the primer in the range between 60% and 70% GC. Single-base substitutions of a primer altered amplification, providing new polymorphisms. The size of amplified DNA was mostly between 0.42kbp and 4.3kbp with the most common bands at 1.lkbp. Ten rice were assayed with 78 primers which generated 221 (67.4%) polymorphic amplified DNAs or RAPDs. It was, thus, demonstrated that RAPDs were useful polymorphisms in rice and superior to RFLPs for their technological simplicity. RAPDs would be good alternatives for the construction of a genetic map because of the higher frequencies of polymorphism detection.