벼 RAPD 표지 이용 低溫幼苗生育관여 量的形質遺傳子座(QTL) 分析

Analysis of a QTL associated with rice seedling growth at low temperature using RAPD markers

The purpose of this study was to detect and map quantitative trait loci(QTL) related to rice seedling growth at low temperature. The F₂ populations of 94 plants was obtained from a cross between a coldsusceptible indica cultivar ‘Dular’ and a cold-tolerant japonica cultivar ‘Toyohatamochi’. A molecular genetic map was constructed using random amplified polymorphic DNA (RAPD) markers and QTL associated with the cold tolerance of rice seedling at 18℃ was analyzed using MAPL-MQTL computer program. Analysis of RAPD patterns regarding to cold tolerance in F₂ population of Dular/Toyohatamochi cross indicated that two RAPD markers 20 and 29 were located with 21 cM apart on chromosome No. 5 and QTL related to seedling growth at 18℃ was closely associated with these DNA markers. The association of the RAPD markers on chromosome 5 with the seedling growth at 18℃ was observed in a separate analysis using F₃ plants of the cold-susceptible indica cultivar ‘K-sen 4’ and the cold-tolerant javanica cultivar ‘Silewah’.