枸杞子 突然變異 系統의 作物學的 特性과 RAPD로 본 遺傳的 變異

Variation of agronomic characters and RAPD in induced boxthorn(Lycium chinense) mutants

To obtain the good quality, disease resistance and high yield of Boxthorn cultivar, seeds of cultivar in Chongyang native were treated with gamma rays of ⁶⁰Co 3KR and 6KR, and also the stem and seed of Yuseong 2 were treated with gamma ray of ⁶⁰Co 3KR, respectively. For mutant strains originated from different treatments, growth characteristics, yield and genetic difference using RAPD analysis were investigated. The mutations observed were changes in stem length, leaf area, numbers of branches and 100 seed weight, stem diameter, disease and pest resistance of mutant strains. Fruit setting and yield potential were abundant in mutated seeds and stem of Yuseong 2. On the other hand we have performed polymerase chain reactions on six chromosomal DNA using by forty random primers and followed by RAPD fragments. And also we selected ten primers which amplified to chromosomal DNAs and its PCR-DNA band size were below to 2.0 bp. To konw genetic similarity, we analysis polymorphisms of PCR-DNA bands by statistics, and its genetic difference distance were 0.7~1.0. The Boxthorn of mutant strains treated with Co⁶⁰ were discussed with morphological feature and RAPD-DNA bands.