밀×야생밀 속간잡종의 genomic in situ hybridization

Genomic in situ hybridization in intergeneric hybrids between wheat and some wheatgrasses

Genomic in situ hybridization (GISH) was used to discriminate parental chromosomes of 3 intergeneric hybrids such as Triticum aestivum×Thinopyrum junceum, T. aestivum×Th. ponticum and T. aestivum×Leymus karelinii. Biotin labelled T. aestivum DNA was hybridized to the chromosomes of the three hybrids. In the hexaploid (2n=6x=42) T. aestivum×Th. junceum, 21 chromosomes with origin of T. aestivum were observed yellow-green, and the rest were orange-red. 21 yellow-green chromosomes and 35 orange-red ones were observed in the octaploid (2n=8x=56) T. aestivum×Th. ponticum, while the heptaploid (2n=7x=47) T. aestivum×L. karelinii was clearly distinguished as having 21 yellowgreen and 28 red chromosomes. Some of 35 orange-red chromosomes of Th. ponticum have bands with yellow-green, indicating that some regions of chromosomes of Th. ponticum were hybridized with T. aestivum DNA used as probe. The results showed that GISH could identify chromosomes of different origin from the hybrids.