토양으로부터 α-glucosidase 저해제 생산균의 분리 및 특성

Isolation and Characterization of Bacteria Producing α-Glucosidase Inhibitor from Soil

The isolation, characterization and culture conditions of bacteria producing α-glucosidase inhibitor from soil and Korean fermented foods were investigated. As a result of screening for the α-glucosidase inhibitory activity on 100 bacteria isolated, strain N1-5 with an inhibition ratio of 71.6% against α-glucosidase activity compared with the inhibition ratio of acarbose(25μg/ml) of 49.1%, was finally selected. This strain was identified by Vitek2 compact system and 16S rDNA sequencing, and named Bacillus subtilis N1-5. The optimum carbon source of B. subtilis N1-5 for the production of α-glucosidase inhibitor was 1% glucose and the optimum nitrogen source was 0.6% polypeptone. The production of α-glucosidase inhibitor by B. subtilis N1-5 showed a typical primary metabolite production pattern in the 5-liter jar fermenter using optimized media. The partially purified α-glucosidase inhibitor by B. subtilis N1-5 was maintained at pH 2.0~3.0 without significantly changing α-glucosidase inhibitory activity. From the above results, it was confirmed that B. subtilis N1-5 isolated from soil produces a relatively stable α-glucosidase inhibitor similar to acarbose, a therapeutic agent for diabetes.