Yersinia enterocolitica의 增菌培地에 관하여

Studies on the Enrichment Media of Yerstnia enterocolitica

The enrichment media are necessary to euhance the growth of desirable organisms and to supress the growth of undesirable bacteria in specimens. Phosphate-buffered solution has been used for enrichment medium to isolate Yersinia enterocolitica, but it needs too long period of incubation to enrich this organism in specimen. Salmonella-Shigella (SS) agar has been used as selective or differential medium for enteric pathogens. Since this medium contains components which inhibit the growth of coliforms, it is reasonable to suppose that the formula of SS agar is effective for enrichment of Yersinia in feces. SS broth and MacConkey broth were prepared by filtering out the agar in solubilized SS agar and MacConkey agar, and the multiplication of Yersinia and E. coli in these media were tested at 15℃, 21℃ or 37℃. The growth of Yersinia in SS broth and MacConeky broth were superior to E. coli until 24 hours at 15℃, but the growth was not sufficient to isolate. At 21℃ of growth temperature, while the growth of Yersinia in MacConkey broth was inferior to E. coli, the enrichment of Yersinia in SS broth was very superior to E. coli as well as in SS broth contained 10 percent of swine feces for 18 and/or 24 hours.