Campylobacter jejuni와 campylobacter coli의 감별분류를 위한 hippurate 가수분해, H₂S 생성 및 triphenyltetrazolium chloride시험에 대한 검토

Examination on the huppurate hydrolysis, H₂S production and triphenyltetrazolium chloride test for the differentiation of campylobacter jejuni and campylobacter coli

A test of hippurate hydrolysis, H₂S production in TSI medium and a test of tolerence of triphenyltetrazolium chloride(TTC) were studied of 103 C. jejuni and 78 C. coli strains isolated from animal feces and reference strains to determine their usefulness for differentiation of C. jejuni and C. coli. The results were summerized as follows. Eighty-two decimal five percentage of the 103 C. jejuni strains hydrolysed sodium hippurate. Ability to hydrolysed hippurate were seen in 92.9% reference strains, 92.6% of cattle isolates, 85.7% of chicken isolates, 75.3% of swine isolates and 75.3% of duck isolates. Ninety-one percentage of the 78 C. coli strains were H₂S production in TSI medium, but 103 C. jejuni were negative. Ability to H₂S production in TSI medium for C. coli strains were seen in 100% of cattle isolates, 100% of chicken isolates, 92.6% of swine isolates and 82.6% of duck isolates. Tolerence to 0.8, 1.0 and 1.2mg of TTC per ml for 103 C. jejuni were seen 66.1%, 28.2% and 12.5%, whereas 77 C. coli were seen 100%, 96.2% and 92.0%, respectively.