계태아뇨막강액을 첨가한 계태아 섬유아단층세포에서의 newcstle disease virus의 증식성

Propagation of newcastle disease virus in chicken embryo fibroblast monolayer supplemented with chicken embryo allantoic fluid

Effect of chicken embryo allantoic fluid on the propagation of NDV in CEF culture was studied. Physico-chemical characteristics of chicken embryo allantoic fluid were also investigated. The experimental results were summarized as following: 1. The propagation of NDV(B₁) in CEF culture was promoted by the supplementation of chicken embryo allantoic fluid to the culture medium. 1) Log10 TCID50 0.1ml of NDV(B₁) propagated in embryonated egg was 9.0±1.0 in the CEAF supplemented group and 1.3±0.3 in the control group. 2) Log₂ HA titers of NDV(B₁) propagated in CEF cultures were 7.5±0.5(released and cell-bound virus), 4.0±1.0(released virus) and 8.0±1.0(cell-bound virus) in the CEAF supplemented group. But those of control groups were 4.5±0.5, 0.0, and 4.5±0.5 respectively. 3) Log10 EID50 /0.1ml of NDV(B₁) propagted in CEF culture was 9.3 in the CEAF supplemented group and 3.8 in the control group. 2. Chicken embryo allantoic fluids harvested from 10, 11, or 12-day-old embryonated eggs were suitable for the propagation of NBV(B₁) in CEF culture. 3. The amount of chicken embryo allantoic tluid suitable for the propagation of NDV(B₁) in CEF culture was 5~10%.