돼지의 백혈구 인터페론 생산에 관한 비교연구

Comparative study on the production of interferons from porcine blood leukocytes

Attempts were to produce porcine leukocyte interferon(PorLeIF) and porcine immune interferon(PorIIF) in the culture of porcine leukocytes. The interferons produced were tested for antiviral activity against vesicular stomatitis virus on porcine─derived PK(15) cells, human─derived FL cells, and korean native black goat─derived BGK cells. The results were summarized as follows: 1. In the isolation of porcine leukocytes, the mean isolation rate by the buffy coat separation method(28.7%) was higher than that by the hydroxyethyl starch─RBC sedimentation method(9.2%). 2. When NDV(BI)─induced PorLeIFs were assayed on PK(15) cells, the mean titers were 129 IU/ml and 72 IU/ml respectively, being 55.8% of the activity in homologous species system expressed in heterologous system. 3. The activities of PHAㆍP─induced PorIIFs were 197 IU/ml on PK(15) cells and no activity on human FL cells. The mean antiviral activity of PorIIF was 1.5 times that of PorLeIF in PK(15) cells. 4. The cytopathic effect of vesicular stomatitis virus was observed in BGK cells derived from Korean native black goat kidney permitting interferon assay on the cells. While the cross-species antiviral activity of reference human α, β-interferon was observed on the cells, PorLeIF and PorIIF did not show any activity.