Campylobacter장염에 대한 역학적 연구 2. 분리주의 항균물질에 대한 감수성과 plasmid 유형

Epidemiological studies on compylobacter enteritis in Korea 2. Antimicrobial suscptibility and plasmid patterns of campylobacter jejuni isolated from animals

Drug-resistant strains isolated from animals have have become an increasingly serious problem because of their resistance to antimicrobial agents and as potential donors of their R-Plasmid to sensitive strains. A total of 129 strains of C. jejuni isolated from pigs and chickens were examined for the susceptibility to 10 antimicrobial agents. Minimal inhibit-ory concentration(MIC) ranges of antimicrobial agents to C. jejuni isolates were○0.2 to 12.5㎍/㎖ for gentamicin and chloramphenicol, ○0.2to25㎍/㎖ for kanamycin and nalidixic acid 0.2 to○400㎍/㎖ for streptomycin. Resistance rates of C. jejuni were showed to in order of cephalothin(100%) rifampin(86%), streptomycin(74.4%), tetracycline(58.1%), erythromycin(24.8%) and ampicillin(3.1%), all of the strains were sensitive to chloramphenicol, gentamicin and kanamycin. The drug resistance patterns of 87 chicken isolates to C. jejuni to 10 antimicrobial agents were showe different patterns, and Cep Sm Re Tc pattern and Cep Sm Re pattern were relatively common, and also 42 pig isolates were showed 5different patterns and Cep Sm Tc pattern were most frequeint and Cep Sm Re Tc pattern were most frequent. A total of 57 isolates of C. jejuni were subjected to test for the presence of plasmid DNA of the isolates examined. 68.4% of the isolates were noted to harbor plasmid DNA. Plasmid occurence rate from pigs and chickens were 70.6% and 62.%, respectively. The strain with a large molecular weight plasmid, ranging from 30-78Md were identified as tetracycline resistant.